A novel pair of inducible expression vectors for use in Methylobacterium extorquens
نویسندگان
چکیده
منابع مشابه
dehydrogenase from Methylobacterium extorquens
The structure of methanol dehydrogenase (MDH) at 0.194 nm (1.94 A) has been used to provide a model structure for part of a membrane quinoprotein glucose dehydrogenase (GDH). The basic superbarrel structure is retained, along with the tryptophandocking motifs. The active-site regions are similar, but there are important differences, the most important being that GDH lacks the novel disulphide r...
متن کاملmoxB Mutants of Methylobacterium extorquens AMlrif
In Methylobacterium extorquens AMI, genes encoding methanol dehydrogenase polypeptides are transcriptionally regulated in response to Cl compounds, including methanol (M. E. Lidstrom and D. I. Stirling, Annu. Rev. Microbiol. 44:27-57, 1990). In order to study this regulation, a transcriptional fusion has been constructed between a j-galactosidase reporter gene and a 1.55-kb XoI-Sall fragment of...
متن کاملMolecular analysis of mxbD and mxbM, a putative sensor-regulator pair required for oxidation of methanol in Methylobacterium extorquens AM1.
Five genes are thought to be required for transcription of methanol oxidation genes in Methylobacterium strains. These putative regulatory genes include mxcQE, which encode a putative sensor-regulator pair, and mxbDM and mxaB, whose functions are less well-understood. In this study, mxbDM in Methylobacterium extorquens AM1 were shown to be required for expression of a xylE transcriptional fusio...
متن کاملXoxF is required for expression of methanol dehydrogenase in 1 Methylobacterium extorquens AM 1 2 3
XoxF is required for expression of methanol dehydrogenase in 1 Methylobacterium extorquens AM1 2 3 Elizabeth Skovran, Alexander D. Palmer, Austin M. Rountree, Nathan M. Good and 4 Mary E. Lidstrom 5 Department of Chemical Engineering and Department of Microbiology 6 University of Washington 7 Seattle, WA 98195-2180 8 9 Submission Date: 5-20-11 10 11 12 * Corresponding author: Mailing address: 6...
متن کاملMetabolic engineering of Methylobacterium extorquens AM1 for 1-butanol production
BACKGROUND Butanol is a promising next generation fuel and a bulk chemical precursor. Although clostridia are the primary industrial microbes for the fermentative production of 1-butanol, alternative engineered hosts have the potential to generate 1-butanol from alternative carbon feedstocks via synthetic metabolic pathways. Methylobacterium extorquens AM1, a facultative methylotrophic α-proteo...
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ژورنال
عنوان ژورنال: BMC Research Notes
سال: 2013
ISSN: 1756-0500
DOI: 10.1186/1756-0500-6-183